An RNA Conformational Switch Regulates Pre-18S rRNA Cleavage
نویسندگان
چکیده
منابع مشابه
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Numerous factors direct eukaryotic ribosome biogenesis, and defects in a single ribosome assembly factor may be lethal or produce tissue-specific human ribosomopathies. Pre-ribosomal RNAs (pre-rRNAs) must be processed stepwise and at the correct subcellular locations to produce the mature rRNAs. Nop9 is a conserved small ribosomal subunit biogenesis factor, essential in yeast. Here we report a ...
متن کاملBase pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.
The loop of a stem structure close to the 5' end of the 18S rRNA is complementary to the box A region of the U3 small nucleolar RNA (snoRNA). Substitution of the 18S loop nucleotides inhibited pre-rRNA cleavage at site A(1), the 5' end of the 18S rRNA, and at site A(2), located 1.9 kb away in internal transcribed spacer 1. This inhibition was largely suppressed by a compensatory mutation in U3,...
متن کامل18S rRNA processing requires the RNA helicase-like protein Rrp3.
We report the identification of a new gene, RRP3 (rRNA processing), which is required for pre-rRNA processing. Rrp3 is a 60.9 kDa protein that is required for maturation of the 35S primary transcript of pre-rRNA and is required for cleavages leading to mature 18S RNA. RRP3 was identified in a PCR screen for DEAD box genes. DEAD box genes are part of a large family of proteins homologous to the ...
متن کاملfrom 18S rRNA Sequences
Several families in the beetle suborder Adephaga have an aquatic life style and are commonly grouped in the “Hydradephaga,” but their monophyly is contentious and relationships between and within these families are poorly understood. Here we present fulllength 18S rRNA sequence for 84 species of Hydradephaga, including representatives of most major groups down to the tribal level, and a total o...
متن کاملAn RNA spiking method demonstrates that 18S rRNA is regulated by progesterone in the mouse uterus.
Identifying suitable housekeeping genes for quantitative RT-PCR in the uterus is problematic, as this tissue undergoes significant structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of 18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an 'RNA spike' standard into the reverse transcripti...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2011
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2010.09.064